tag:blogger.com,1999:blog-6914086676454800973.post2535249784366266072..comments2023-10-29T01:46:00.188-08:00Comments on VeggiePharm: Mass Production of AntibioticsTim Steelehttp://www.blogger.com/profile/01611027687223434753noreply@blogger.comBlogger5125tag:blogger.com,1999:blog-6914086676454800973.post-27957173949458241702015-03-11T22:35:12.288-08:002015-03-11T22:35:12.288-08:00Interesting blog posted..............Interesting blog posted..............Buy Generic Ciprohttp://www.pharmaonlinerx.com/antibiotics/28-generic-cipro.htmlnoreply@blogger.comtag:blogger.com,1999:blog-6914086676454800973.post-36232128578979019422014-10-16T09:19:01.856-08:002014-10-16T09:19:01.856-08:00And, Alexandra, I am a fan of yours already, too! ...And, Alexandra, I am a fan of yours already, too! A well-cited, easy-to-read excerpt is not easy to pull off in a Blogger comment.<br /><br />Perfect, thanks!<br /><br />This makes me wonder if it could change the nature of fecal transplants, especially the DIY types. <br /><br />There was much talk a while back about a paper from the 1940's that talked about WWII soldiers in Saharan Africa eating freshly deposited camel poop as a cure for dysentery, learned by watching the locals. It's been written about here: <br /><br />http://www.rense.com/general4/bac.htm<br /><br /><br /><br />Tim Steelehttps://www.blogger.com/profile/01611027687223434753noreply@blogger.comtag:blogger.com,1999:blog-6914086676454800973.post-57789337463138802312014-10-16T00:16:34.084-08:002014-10-16T00:16:34.084-08:00"Preparation of Frozen Inocula
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Processing w..."Preparation of Frozen Inocula<br />.<br />Processing was carried out under aerobic conditions. A fecal suspension was generated in normal saline without preservatives using a commercial blender. Materials were sequentially sieved to remove particulate material. The final slurry was concentrated by centrifugation and resuspended in saline at one-tenth the volume of the initial sample with 10% glycerol added as a bacterial cryoprotectant. Fecal matter solution was pipetted into size 0 capsules (650 µL), which were closed and then secondarily sealed in size 00 capsules. Capsules were stored frozen at −80°C (−112°F). One to two hours prior to administration, they were transferred to −20°C, then transported to the clinic on dry ice. Commercially available acid-resistant hypromellose capsules (DRCaps, Capsugel) were used. Stability of capsules in an acid environment mimicking the stomach was tested internally by evaluating trypan blue–filled capsules. At 37°C (99°F) and a pH of 3 or less, the capsules were stable for 115 minutes before dye was released. These results are comparable with data published after we conducted our internal evaluation.20 Each inoculum was prepared from the feces of a single donor and a full treatment of 30 capsules contained sieved, concentrated material derived from a mean of 48 g of fecal matter (mean per capsule, 1.6 g; range, 1.0-2.05 g)."<br /><br />http://jama.jamanetwork.com/article.aspx?articleid=1916296<br />Oral, Capsulized, Frozen Fecal Microbiota Transplantation for Relapsing Clostridium difficile Infection<br /><br />Love the blog (already a follower ;-) ) and just wanted to mention: you have the best blog list ever! Just one look and I know where to go to for new stuff. Thanks!<br /><br />Alexandra<br />Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-6914086676454800973.post-43052947744820433822014-10-15T21:22:37.635-08:002014-10-15T21:22:37.635-08:00Hmmm. This is interesting, and very new! Fecal p...Hmmm. This is interesting, and very new! Fecal pills seemed to fizzle out last year with less-than-stellar reviews. This new paper mentions 'frozen' fecal capsules. <br /><br />I wonder, do they mean 'freeze-dried' or actual 'frozen poop'? And, how do they encapsulate it to ensure it opens in the colon?<br /><br />I remember several papers that used RS for encapsulation since it isn't degraded until it hits the large intestine.<br /><br />Thanks, Gemma! Tim Steelehttps://www.blogger.com/profile/01611027687223434753noreply@blogger.comtag:blogger.com,1999:blog-6914086676454800973.post-173644742520824702014-10-15T02:40:42.900-08:002014-10-15T02:40:42.900-08:00Wow!
"Fecal capsules resolve Clostridium di...Wow! <br /><br />"Fecal capsules resolve Clostridium difficile infection in 90% of patient"<br /><br />http://www.medicalnewstoday.com/articles/283766.phpGemmahttps://www.blogger.com/profile/18051515271519007652noreply@blogger.com